PROMOTING SCIENCE AND TECHNOLOGY BETWEEN INDIA AND THE U.S.
2016 Khorana Scholars
Sai Sinhu is working on Biosensors for small molecules based on Transcription Factors. They have transcription factor gene and a GFP transformed into a bacterial cell. So when the transcription factor goes and binds to promoter of GFP , the GFP is repressed. When the small molecule binds to transcription factor, it leaves the promoter making space for RNA polymerase to bind and GFP is expressed. So this GFP on Off helps us to sense how much small molecule is produced in the cell. I am checking which promoter for transcription factor will give us a good on-off signal. So they selected promoters which have diverse distributed protein production units and planning to transform them into bacterial cells and see how the GFP on off works.