Host: Indian Institute of Science Bangalore
Bhagyashri is working with a team of pHD scholars under the guidance of Dr. Ramanan to clone sets of four sgRNAs into single lentiviral vectors utilizing Golden Gate assembly. Using multiplex CRISPR/Cas9 genome editing, these clones would be further used to create transgenic knockout mice, since the sgRNAs are specifically designed to complement genes that may be responsible for reactive astrocytes. This process will help identify the pathways, genes, and proteins responsible for neuroprotective astrogliosis.