The Klein Lab discovered that amyloid-beta oligomers (AβOs) causes neuronal death and ultimately dementia in Alzheimer’s disease. My research was on understanding the cellular and subcellular localization of AβOs in isolated CNS neurons. This was done by isolating neurons from chick embryo retina and performing immunocytochemistry to check the distribution and localization of AβOs . AβOs were found mainly in ganglion cells and subcellularly found in cell body and processes of the neurons.
2018 Khorana Scholar who went to Northwestern University and worked under William Klein.
My main project is “Studies of brain cell loss caused by the toxic amyloid beta oligomers linked to Alzheimer’s dementia.” I am working on two things: one is to study the role of amyloid beta oligomers in the CNS development; the other one is to confirm the binding of synthetically produced fluorescent peptides with the amyloid beta oligomers.
2018 Khorana Scholar who worked at Northwestern University under Professor Yuan Yang. Nirvik is looking into the investigation of detection threshold for transcutaneous electrostimulation in CVA patients and healthy individuals- a comparative study of the effects of gender, age and subcutaneous fat.
Tejaswini was researching biochemistry and molecular biology of Alzheimer’s disease at Department of Neurobiology, Klein Lab, Northwestern University, Illinois. The unifying aim of her lab was to ‘Make Alzheimer’s a Memory!’ Alzheimer’s is a current pressing ailment worldwide, numbers expected to rise to 70 million by 2030. Her lab had discovered the Amyloid Beta Oligomers, the bad guys of AD, in 1998. Broadly put, Tejaswini’s work was centered around arresting Abeta Oligomers’ fibrillogenesis by chemically cross-linking and hence stabilizing them, thereby preventing the formation of Amyloid fibrils. She will be using immunotechniques like High Precision Coomassie Assay, Western Blot, Silver Staining, Immunofluorescence Assays to detect and quantify AbO.