Biology, Earth Sciences
We use zebrafish, a small fresh water tropical fish endemic to the Ganges, as our model system. The embryonic and larval stages of these fish are transparent allowing for direct visual observation of developing internal organs including the brain. We employ a suite of techniques to tease out the circuitry responsible for generating swimming in developing and more mature zebrafish. We record electrical activity from individual spinal and brain neurons using extracellular and whole-cell patch clamp techniques. We record activity from populations of neurons simultaneously using calcium imaging. We generate transgenic zebrafish to express proteins of interest in particular neurons. This allows us to selectively ablate and also to electrically activate/inactivate specific populations at will. Using these cutting edge tools and technologies, we hope to throw light on the development of neural circuits and the neural basis of locomotion.